4 6 diamidino 2 phenylindole (Dojindo Labs)
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4 6 Diamidino 2 Phenylindole, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 495 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+solution/Cellstain+DAPI+solution/pm42863997-63-16-24
Average 96 stars, based on 495 article reviews
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other:Article Title: MicroRNA-33 inhibition ameliorates muscular dystrophy by enhancing skeletal muscle regeneration Article Snippet: Article Title: Trajectory analysis reveals an uncommitted neuroblastic state in MYCN-driven neuroblastoma development. Article Snippet: They were then incubated with rabbit anti-MYCN (D1V2A) antibody (1:100, 84406, Cell Signaling Technology) in antibody diluent (1% BSA in 0.3% Triton X-100/D-PBS) overnight at 4 °C. Article Title: Inhibiting KRAS with CD47 and immune checkpoint overcomes intrinsic resistance to combined KRAS and immune checkpoint inhibitor therapy Article Snippet: DAPI solution , Article Title: Spatial proteomics reveals prefrontal circuit diversity in socioemotional behaviour Article Snippet: The following antibodies were used: rabbit anti-HA (Cell Signaling Technology, C29F4), rabbit anti-Homer1 (Synaptic Systems, 160-003), guinea pig anti-VGLUT-1 (Synaptic Systems, 135-304), monoclonal α-Tubulin (Santa Cruz, sc-32293), rabbit anti-KIAA0100 (BLTP2) (MyBioSource, MBS8243055), rabbit anti-mCherry (Abcam, ab167453), rabbit anti-Neurexin-1α (Nittobo Medical, MSFR104630), chicken anti-GFP (Merck, AB16901), Alexa Fluor 488 Goat anti-Mouse (ThermoFisher, A32723), Alexa Fluor 488 Goat anti-Rabbit (ThermoFisher, A-11008), Alexa Fluor 488 Goat anti-Guinea pig (ThermoFisher, A11073), Alexa Fluor 488 Goat anti-Chicken (ThermoFisher, A-11039), Alexa Fluor 555 Goat anti-Rabbit (ThermoFisher, A21428), Alexa Fluor 555 Goat anti-Guinea pig (ThermoFisher, A21435), Alexa Fluor 647 Donkey anti-rabbit (ThermoFisher, A31573), Alexa Fluor 647 Goat anti-Chicken (ThermoFisher, A-21449), Alexa Fluor 647 Donkey anti-Guinea pig (Jackson ImmunoResearch, 706-605-148), Alexa Fluor 647 Streptavidin (ThermoFisher, S21374), StarBright Blue 700 anti-Mouse (BioRad, 12004159), StarBright Blue 700 anti-Rabbit (BioRad, 12004162), CF770 anti-Mouse (Biotium, 20077), CF770 anti-Rabbit (Biotium, 20078) and Staining:Article Title: Thiol methyltransferase METTL7B coordinates lipid metabolism and promotes tumor progression in pancreatic cancer Article Snippet: Samples were then incubated at 4°C overnight with primary antibodies against METTL7B (Atlas Antibodies, HPA038644, 1:200 dilution), PDIA3 (Sigma-Aldrich, AMAB90988, 1:200 dilution), or CD29 (BD, 610467, 1:200 dilution) diluted in BSA-T. After washing three times with PBS-T, samples were incubated at room temperature for 1 hour with Alexa Fluor 488-labeled anti-rabbit IgG secondary antibody (Thermo Fisher Scientific, A21206, 1:1000 dilution), Alexa Fluor 555-labeled anti-mouse IgG secondary antibody (Thermo Fisher Scientific, A31570, 1:1000 dilution), or Alexa Fluor 647-labeled anti-mouse IgG secondary antibody (Thermo Fisher Scientific, A31571, 1:1000 dilution) diluted in BSA-T, followed by three washes with PBS-T. For LD staining, samples were incubated with 0.1 μM Lipi-Blue (Dojindo, LD01) or Lipi-Deep Red (Dojindo, LD04) in PBS at 37°C for 1 hour. .. For nuclear staining, samples were incubated with 1 μg/mL Article Title: Nuclear enlargement after confined migration in cancer cells Article Snippet: .. The cells were then washed again with PBS and stained with Incubation:Article Title: Thiol methyltransferase METTL7B coordinates lipid metabolism and promotes tumor progression in pancreatic cancer Article Snippet: Samples were then incubated at 4°C overnight with primary antibodies against METTL7B (Atlas Antibodies, HPA038644, 1:200 dilution), PDIA3 (Sigma-Aldrich, AMAB90988, 1:200 dilution), or CD29 (BD, 610467, 1:200 dilution) diluted in BSA-T. After washing three times with PBS-T, samples were incubated at room temperature for 1 hour with Alexa Fluor 488-labeled anti-rabbit IgG secondary antibody (Thermo Fisher Scientific, A21206, 1:1000 dilution), Alexa Fluor 555-labeled anti-mouse IgG secondary antibody (Thermo Fisher Scientific, A31570, 1:1000 dilution), or Alexa Fluor 647-labeled anti-mouse IgG secondary antibody (Thermo Fisher Scientific, A31571, 1:1000 dilution) diluted in BSA-T, followed by three washes with PBS-T. For LD staining, samples were incubated with 0.1 μM Lipi-Blue (Dojindo, LD01) or Lipi-Deep Red (Dojindo, LD04) in PBS at 37°C for 1 hour. .. For nuclear staining, samples were incubated with 1 μg/mL Article Title: Trajectory analysis reveals an uncommitted neuroblastic state in MYCN-driven neuroblastoma development Article Snippet: They were then incubated with rabbit anti-MYCN (D1V2A) antibody (1:100, 84406, Cell Signaling Technology) in antibody diluent (1% BSA in 0.3% Triton X-100/D-PBS) overnight at 4 °C. .. This was followed by incubation with an Alexa Fluor 594-labeled goat anti-rabbit IgG secondary antibody (1:500, A-11012, Thermo Fisher Scientific) and |

